| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lopez, Rylie A (18)
|
PNBBST |
4:49.97 |
4:18.88 |
-31.09 |
| 2 |
Lebl, Ilektra (22)
|
ILUN-I |
4:54.48 |
4:20.09 |
-34.39 |
| 3 |
Cornish, Kristin A (17)
|
MRBAD |
4:50.97 |
4:22.62 |
-28.35 |
| 4 |
Palmer, Lydia M (17)
|
MASJAC |
4:50.41 |
4:22.64 |
-27.77 |
| 5 |
Lynch, Meghan E (17)
|
CTGYWD |
4:53.19 |
4:23.64 |
-29.55 |
| 6 |
Benda, Caroline F (17)
|
MDNBAC |
4:55.51 |
4:24.86 |
-30.65 |
| 7 |
Boszormenyi, Bettina (23)
|
FLUN-1 |
4:55.36 |
4:29.13 |
-26.23 |
| 8 |
Frank Rodriguez, Nicole (17)
|
FGAZFL |
4:59.91 |
4:29.84 |
-30.07 |
| 9 |
Bagnall, Morgan P (17)
|
CTBULL |
5:01.91 |
4:30.10 |
-31.81 |
| 10 |
O'Neill, Lucy E (17)
|
SECMSA |
4:58.29 |
4:30.43 |
-27.86 |
| 11 |
Hervey, Lauren K (18)
|
MVCSC |
5:05.05 |
4:33.76 |
-31.29 |
| 12 |
Monahan, Grace M (17)
|
HIKSC |
5:01.06 |
4:33.78 |
-27.28 |
| 13 |
Allen, Aisling N (17)
|
ARHAWG |
5:05.98 |
4:34.08 |
-31.90 |
| 14 |
Neely, Cassidy E (18)
|
FLAAC |
5:06.10 |
4:34.31 |
-31.79 |
| 15 |
Smyers, Molly E (17)
|
AMRXA |
5:03.12 |
4:34.58 |
-28.54 |
| 16 |
McKee, Gabrielle E (18)
|
MATOPS |
5:07.92 |
4:34.92 |
-33.00 |
| 17 |
Macmullan, Kira E (17)
|
MDEST |
5:01.25 |
4:37.48 |
-23.77 |
| 18 |
Rome, Norah K (17)
|
CTBULL |
5:07.55 |
4:40.88 |
-26.67 |
| 19 |
Denny, Britney L (17)
|
VACGBD |
5:10.10 |
4:43.80 |
-26.30 |
| 20 |
McCallie, Sydney E (18)
|
VAQSTS |
5:06.31 |
4:47.66 |
-18.65 |